c-jun transcription factor Search Results


kinases jun transcription factor ap 1 junb junb proto oncogene kegg kyoto encyclopedia  (MedChemExpress)
92
MedChemExpress kinases jun transcription factor ap 1 junb junb proto oncogene kegg kyoto encyclopedia
Kinases Jun Transcription Factor Ap 1 Junb Junb Proto Oncogene Kegg Kyoto Encyclopedia, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jun  (Proteintech)
93
Proteintech jun
Jun, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti jun  (Boster Bio)
93
Boster Bio rabbit anti jun
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rabbit anti c jun pab  (Proteintech)
96
Proteintech rabbit anti c jun pab
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rabbit polyclonal anti c jun  (Proteintech)
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Proteintech rabbit polyclonal anti c jun
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
Rabbit Polyclonal Anti C Jun, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pavecs  (Boster Bio)
93
Boster Bio pavecs
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
Pavecs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c jun n  (Proteintech)
94
Proteintech c jun n
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
C Jun N, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pure transcription factors (p50 c-jun  (Active Motif)
90
Active Motif pure transcription factors (p50 c-jun
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
Pure Transcription Factors (P50 C Jun, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transcription factor activity assay kit  (RayBiotech inc)
90
RayBiotech inc transcription factor activity assay kit
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
Transcription Factor Activity Assay Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c-jun transcription factor  (Inserm Transfert)
90
Inserm Transfert c-jun transcription factor
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
C Jun Transcription Factor, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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targeting to the oncogenic transcription factor c-jun protein  (Affibody)
90
Affibody targeting to the oncogenic transcription factor c-jun protein
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
Targeting To The Oncogenic Transcription Factor C Jun Protein, supplied by Affibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c-jun transcription factor  (Lallemand inc)
90
Lallemand inc c-jun transcription factor
A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with <t>polyclonal</t> anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.
C Jun Transcription Factor, supplied by Lallemand inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with polyclonal anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.

Journal: Cell chemical biology

Article Title: Divergent JNK Phosphorylation of HDAC3 in Triple Negative Breast Cancer Cells Determines HDAC Inhibitor Binding and Selectivity

doi: 10.1016/j.chembiol.2017.08.015

Figure Lengend Snippet: A) Western blot analysis of MCF-7 cells labeled in situ with photomate (lane 1 and 4), photomate and SAHA (lane 3 and 5) or DMSO (lane 6), reacted with biotin azide tag, and enriched with streptavidin coated magnetic beads. Blot shows enriched fraction eluted off beads and 2.5% of input recognized by anti-HDAC1, 2 and 3 antibodies. B) Graph of the abundance of each class I HDAC in MCF-7 cells quantified by comparison of MCF-7 lysate to a standard curve of each recombinant class I HDACs; visualized by Western blot (Figure S1). C) MS/MS analysis of photomate enriched fractions, as in A). Pie chart on the right shows total number of proteins identified by MS/MS and the number that were specifically enriched when compared to a DMSO control and were decreased by at least 50% when co-treated with SAHA. On the left, bioinformatic annotation of enriched proteins. All specifically enriched proteins are shown in Table S1. D) Gel based visualization of MCF-7 cells labeled with photomate in situ followed by fractionation into cytosol (right) and nucleus (left). Probe staining is shown above, and antibody recognition of the same gel is shown below. Gels are in grey scale for clarity. E) Western blot analysis of HDAC3 co-immunoprecipitates. MDA-MB-231 cells, or MCF-7 cells were lysed and enriched with polyclonal anti-HDAC3 (Abcam) or rabbit IgG protein A bead conjugates (Lanes 2 and 3 respectively). Eluates were analyzed by western blot along with 10% of Input (Lane 1) and blotted with antibodies for complex components. F) Small interfering RNA knockdown of NCOR1 in MCF-7 cells. MCF-7 cells were transfected with siNCOR1 or siControl, followed by labeling with photomate and electrophoretic separation. Antibody recognition of transfected cells is shown above, followed by gel based visualization of photomate engagement of HDAC1and HDAC3 below. Cells were either incubated with photomate (Lane 1) or photomate and excess SAHA (Lane 2) after transfection. All results are representative of at least 3 independent experiments.

Article Snippet: Rabbit polyclonal anti-c-Jun , Protein Tech , 10024-2-AP.

Techniques: Western Blot, Labeling, In Situ, Magnetic Beads, Comparison, Recombinant, Tandem Mass Spectroscopy, Control, Fractionation, Staining, Small Interfering RNA, Knockdown, Transfection, Incubation

KEY RESOURCES TABLE

Journal: Cell chemical biology

Article Title: Divergent JNK Phosphorylation of HDAC3 in Triple Negative Breast Cancer Cells Determines HDAC Inhibitor Binding and Selectivity

doi: 10.1016/j.chembiol.2017.08.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-c-Jun , Protein Tech , 10024-2-AP.

Techniques: Virus, Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Blocking Assay